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Image Search Results
Journal: Current protocols in neuroscience
Article Title: Multisite electrophysiology recordings in mice to study cross-regional communication during anxiety
doi: 10.1002/cpns.32
Figure Lengend Snippet: Drive construction. (A1.B1). Designs (constructed in Blender) for 3D printing the stage (A1) and cuff (B1). These designs were optimized for a Neuralynx 16-channel EIB, and are available as Supplementary Material, and at likhtiklab.com/tools (A2,B2.) The 3D printed stage and cuff. Micrcoelectrodes will be carried on the open side of the stage (C.) The 16-channel EIB attached to the stage and cuffs by hex screws (side view). The reference and ground wires are soldered into the board prior to being mounted on the stage. (D.) Top view of the drive at this stage of assembly. Note that there are two hex screws that attach the board to the stage and a third screw that is providing stability. The Ground wire is in the back, and will be soldered to a screw above the cerebellum. The reference wire is in the front and will be soldered to the frontal reference screw. A – anterior, P - posterior. (E.) Microwires are pinned into the EIB and attached to an optic fiber for stability and accuracy of targeting, especially for deeper structures. (F.) The completed drive after all the wires have been covered with dental cement. Arrow shows a channel that was left open for an LFP wire that will be implanted and pinned into the drive during surgery. LFP channels added to the drive during surgery will also be covered up with dental cement. (G.) Additions to recording electrodes can be optic fibers or cannulae. Either of these can be customized to be implanted unilaterally or bilaterally. Arrowheads in 3E and 3F point to electrode bundle attached to an optic fiber.
Article Snippet: Design files for the illustrated stage are provided as a Resource, and are available for download at http://likhtiklab.com/tools/ Transfer the design file to software that interfaces with a
Techniques: Construct